The Ecto-5nucleotidase/CD73 Mediates Leishmania amazonensis Survival in Macrophages

Figure 2

Resident macrophages down regulate CD73 expression in in vitro. Resident macrophages were harvested from naïve C57BL/6 mice. Total peritoneal cell population was counted, and viability of the cells was determined by trypan blue. cells were cultured in vitro at 37°C and was left for 24 h, 48 h, and 72 h of resting and subsequently incubated for 24 h at 33°C/5%CO₂ before analysis by flow cytometry (a). Size and granularity for total peritoneal population (b). F4/80⁺ cells were first gated from total peritoneal population and then cells expressing CD39 and CD73 in F4/80⁺ population from 24 h, 48 h, and 72 h rested macrophages were overlaid in the histograms (c and d). The percentage of cells expressing (e) CD39 and (f) CD73 is represented in bar diagrams for macrophages. This result is representative of 3 independent experiments. indicates the statistical difference using paired two-tailed Student’s -test.

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