The Ecto-5nucleotidase/CD73 Mediates Leishmania amazonensis Survival in Macrophages

Figure 3

Leishmania amazonensis increases CD73 expression but does not alter cytokines and nitric oxide (NO) production. Resident cell population was collected from naïve C57BL/6 mice and rested for 72 h prior to infection as previously discussed in the methodology. The cells were infected with CFSE-tagged metacyclics of L. amazonensis, and additionally, another group was treated with 5 μg/ml of LPS. The cells were further incubated for 24 h at 33°C/5%CO₂. Supernatant from all groups was collected for the measurement of TNF-α, IL-10, and NO. Macrophages (MФ) expressing (a) CD39, (b) CD73, (c) CD39CD73, and immunoassays from culture supernatants for (d) TNF-α, (e) IL-10, and (f) nitric oxide production in treated macrophages are shown in bar diagrams. This result is the of at least 3 independent experiments. indicates the statistical significance between infected and control groups using one-way analysis of variance (ANOVA) followed by Bonferroni posttest.

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