[Retracted] Depletion of Fibroblast Growth Factor 12 Restrains the Viability, Stemness, and Motility of Colorectal Cancer

Figure 2

FGF12 knockdown inhibits the proliferation and stemness of CRC cells and induces apoptosis. (a, b) qRT-PCR and WB analysis. The transfection efficiency of sh-FGF12 and sh-Control (NC) in LoVo and SW480 cells were analyzed via qRT-PCR and WB analysis. (c, d) CCK-8 and EdU assays were performed to evaluate the proliferative ability of CRC cells. (e) The results of flow cytometry showed that FGF12 depletion promoted CRC cell apoptosis. (f) Tumorsphere assay of CRC cells transfected with sh-FGF12 and sh-NC. (g, h) WB analysis showed protein expression of Ki-67, cleaved-caspase 3, NANOG, and OCT4 in sh-NC and sh-FGF12 in LoVo and SW480 cells. CRC: colorectal cancer; , .

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