[Retracted] Depletion of Fibroblast Growth Factor 12 Restrains the Viability, Stemness, and Motility of Colorectal Cancer

Figure 4

FGF12 regulates the proliferation, apoptosis, invasion, and stemness of CRC cells by activating the PI3K/AKT signaling pathway. (a) WB was conducted to detect the expression of PI3K/AKT target genes (p-PI3K, PI3K, p-AKT (473 S), p-AKT (T308), and AKT) in FGF12-silenced cells. (b) WB was conducted to detect the expression levels of PI3K/AKT target genes in FGF12-deficient cells after adding 740 Y-P. (c) EdU assays was performed to evaluate the proliferative ability of FGF12-deficient cells after adding 740 Y-P. (d) Flow cytometry was performed to evaluate the apoptosis of FGF12-deficient cells after adding 740 Y-P. (e) Tumorsphere assay of CRC cells transfected with sh-FGF12 after adding 740 Y-P. (f) Transwell assays were used to evaluate the invasion of CRC cells transfected with sh-FGF12 after adding 740 Y-P. (g) WB was performed to detect the expression levels of EMT markers in FGF12-deficient cells after adding 740 Y-P. CRC, colorectal cancer; , .

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