Preparation of Spice Extracts: Evaluation of Their Phytochemical, Antioxidant, Antityrosinase, and Anti-α-Glucosidase Properties Exploring Their Mechanism of Enzyme Inhibition with Antibrowning and Antidiabetic Studies In Vivo

Figure 3

Mechanism of α-glucosidase inhibition. (a) Lineweaver-Burk plot (LBP) for the inhibition of α-glucosidase in the presence of Star anise ETAC extract. The extract concentrations 0-45 μg/mL; however, pNPG concentrations (0.125 to 1 mM) were used. (b) The insets represent the plot of the slope from LBP versus inhibitor. (c) The secondary replot of the LBP, (-intercept) of versus various concentrations of inhibitor. (d) The Dixon plot of the reciprocal of the initial velocities versus various concentrations of extract as inhibitor. (e) Relationship between the catalytic activity of pNPG and various concentrations of extract.

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