Identification of RALA as a Therapeutic Target and Prognostic Predictor of Osteosarcoma

Figure 2

Molecular network and function of RALA in OS. (a) Molecular interaction network of RALA was analyzed using the STRING tool. (b–e) TOP 5 coexpression genes in different tumors were downloaded from GEPIA2. Correlation between the 5 genes; RALA and ABCE1 expression were presented in sarcoma tissues of TCGA using coexpression heat map (b) and chord diagram (d). Data of OS in TARGET was presented in (c) and (e). ; ; . (f) An intersection analysis of the SND1-binding and correlated genes was conducted. (g, h) Coexpression degree between RALA and ABCE1 in TCGA (g) and TARGET (h) dataset. (i–m) KEGG/GO enrich analyses were performed based on the 50 RALA-binding and 100 interacted genes, containing MF (i), BP (j), CC (k), KEGG pathway (l), and KEGG/GO network (m). (n–s) Functional experiments were performed in MG63 cells. Proliferative ability was detected using EDU assay with representative images (n) and quantitative analysis (q); nuclei stained with DAPI (blue); proliferating cells stained with EDU (red). Migratory ability was detected using wound healing assay with representative images (o) and quantitative analysis (p). Invasive ability was detected using invasion assay with representative images (s) and quantitative analysis (r); cells that pass through Matrigel are stained with 0.2% purple crystal (purple). .

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