Gene Signatures and Associated Transcription Factors of Allergic Rhinitis: KLF4 Expression Is Associated with Immune Response

Figure 3

Profiling of transcription factors associated with immune response on HC and AR. (a) Heatmap shows the overlapping DEGs-dependent immune-related genes () among top library () by performing ChEA3 (https://maayanlab.cloud/chea3/). (b) Barplot shows the frequency of DEGs-dependent immune-related genes binding to predicted transcription factors (). The top 20 genes are indicated. (c) The volcano plot shows the distribution of DEGs. The DEGs-dependent transcription factors are indicated using black arrow. (d) Pairwise correlations for the expression levels of GATA2, KLF4, FOXA3, SPDEF, ZMAT4, and HES4 and enrichment score of monocyte (up) and dendritic cell-related genes (bottom), respectively. (e) Barplot shows the expression levels of KLF4, GATA2, SPDEF, and HES in Ctrl and IL-1β-treated HNEpC cells. The expression levels of each gene were normalized to the expression level of ACTB. The data were presented as the values ( value, Student’s -test). (f, g) GSEA result showing the enriched expression of NF-κB and STAT3-related genes.

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