Gene Signatures and Associated Transcription Factors of Allergic Rhinitis: KLF4 Expression Is Associated with Immune Response

Figure 4

KLF4 transcription factor is associated with immune signature. (a) A genetic network of DEGs-dependent immune response-related genes was constructed showing coexpression (purple), physical interactions (pink), and genetic interactions (green) by using GeneMANIA software in Cytoscape (version 3.9.1). The KLF4 target genes are indicated. (b) The KLF4 transcription factor binding sites of each gene (e.g., CD274, CD44, and IL1RL1) are shown, which are predicted to bind KLF4 by performing AliBaba2.1 (http://gene-regulation.com/pub/programs/alibaba2/index.html) (up). Logos for binding motifs of KLF4 with 9 base pairs (bottom). (c) Barplot shows the expression levels of KLF4 in Ctrl and KLF4-overexpressing HNEpC cells. (d) Barplot shows the expression levels of IL1RL1, CD274, and CD44 in Ctrl and KLF4-overexpressing HNEpC cells. The expression levels of each gene were normalized to the expression level of ACTB. The data were presented as the values ( value, Student’s -test).

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