Neurological Effects of Combining Low Toxic Dose of Tramadol and Nicotine: An Animal Model Evidence of Endoplasmic Reticulum Stress

Table 2

The cycle of real-time PCR for CHOP, BIP, and GAPDH gene expressions.

Initial activation	95 degrees Celsius	12 min
Denaturation	95 degrees Celsius	15 sec
Annealing	62 degrees Celsius	30 sec
Extension	72 degrees Celsius	30 sec