Research Article

Molecular Investigation of the Antitumor Effects of Monoamine Oxidase Inhibitors in Breast Cancer Cells

Table 1

Primers’ forward and reverse sequences with their optimized annealing temperature.

PrimerPrimer sequenceTa (°C)

MAO-AForward: 5-GCCAAGATTCACTTCAGACCAGAG-359
Reverse: 5-TGCTCCTCACACCAGTTCTTCTC-3

Bcl-2Forward: 5-TTGTGGCCTTCTTTGAGTTCGGTG-359
Reverse: 5-GGTGCCGGTTCAGGTACTCAGTCA-3

VEGFForward: 5-CTACCTCCACCATGCCAAGT-359
Reverse: 5-GCAGTAGCTGCGCTGATAGA-3

GAPDHForward: 5-ACAACTTTGGTATCGTGGAAGG-358
Reverse: 5-GCCATCACGCCACAGTTTC-3

Ta: annealing temperature; MAO-A: the gene for monoamine oxidase A enzyme; Bcl-2: the gene for B-cell lymphoma 2; VEGF: the gene for vascular endothelial growth factor; GAPDH: the gene for glyceraldehyde 3-phosphate dehydrogenase.