TRIM9 Interacts with ZEB1 to Suppress Esophageal Cancer by Promoting ZEB1 Protein Degradation via the UPP Pathway

Figure 1

TRIM9 expression was repressed in tumor cells and correlated with worse prognosis for esophageal cancer patients. (a, b) Expression of TRIM9 protein in esophageal tumor tissue and adjacent matched normal tissue samples detected by IHC assay and TRIM9 -score comparison between clinical samples of the esophageal cancer cohort. (c) WB analysis of TRIM9 protein expression among four matched samples of esophageal cancer tumor tissue and surrounding normal tissue. (d) TRIM9 mRNA expression ratio between esophageal cancer tumor tissue and matched adjacent normal tissue. Postsurgical samples were gathered from esophageal cancer patients in our clinical center. (e) TRIM9 mRNA level comparison between esophageal cancer tissue and matched adjacent surrounding tissue collected in our clinical center. (f, g) TRIM9 protein and mRNA levels detected by WB and quantitative RT-PCR experiments in several esophageal cancer cell lines including KYSE-150, KYSE-30, CaES-17, TE-10, NEC, TE-11, TE-1, TE-13, and KYSE-410. (h) Kaplan-Meier plot and log-rank test were utilized to evaluate the overall prognosis of esophageal cancer patients. Two patient groups were divided based on the expression of TRIM9 gene expression compared with the median level.

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