TRIM9 Interacts with ZEB1 to Suppress Esophageal Cancer by Promoting ZEB1 Protein Degradation via the UPP Pathway

Figure 2

TRIM9 suppression aggravates malignancy of esophageal cancer cells. (a) mRNA and protein expression of TRIM9 modulated by TRIM9-specific shRNAs (sh1/sh2) and TRIM9 overexpression plasmid in KYSE-410 and KYSE-30 esophageal cancer cell lines. (b, c) Functional tests on tumor cell migration and invasion capability of KYSE-30/KYSE-410 cell lines transfected with TRIM9 overexpression vectors or TRIM9-specific shRNAs. Percentage of cell migration and invasion of each cell group was calculated and statistically compared. (d–g) Wound healing experiment on KYSE-30/KYSE-410 cell lines transfected with TRIM9-specific overexpression vectors and TRIM9-specific shRNAs, respectively. Percentage of migrated tumor cells in the two cell groups was further calculated and statistically compared. (h, i) Protein and mRNA EMT biomarkers (E-cadherin, N-cadherin, and vimentin) were detected by WB/qRT-PCR experiments. Tumor cell group was transfected with TRIM9-specific shRNAs or overexpression vectors.

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