TRIM9 Interacts with ZEB1 to Suppress Esophageal Cancer by Promoting ZEB1 Protein Degradation via the UPP Pathway

Figure 5

Influence of TRIM9 on ZEB1 degradation velocity in KYSE-410 cells. (a–d) CHX chase assay to evaluate the influence of UPP blockage on TRIM9 and ZEB1 expression in KYSE-410 (a, b) and KYSE-30 (c, d) cell lines. Each cancer cell group was separately transfected with TRIM9-specific shRNAs or TRIM9-specific negative control vectors. (e, f) Co-IP assay to evaluate the impact of TRIM9 expression regulation on ZEB1 protein levels in esophageal cancer cell lines (KYSE-410 and KYSE-30). (g) Each group of tumor cells was treated with MG132 to block the UPP pathway and was synergistically transfected with TRIM9-specific shRNAs or TRIM9-specific overexpression vectors.

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