Detection of Virulence Factors and Antibiotic Resistance among Klebsiella pneumoniae Isolates from Iran

Table 1

PCR primers used in this study.

Target virulence genes	Primer sequence (5-3)	Reference
iucB	F: ATGTCTAAGGCAAACATCGT	[42]
	R: TTACAGACCGACCTCCGTGA
iutA	F: GGGAAAGGCTTCTCTGCCAT	[42]
	R: TTATTCGCCACCACGCTCTT
iroN	F: AAGTCAAAGCAGGGGTTGCCCG	[42]
	R: TGACGCCGACATTAAGACGCAG
fimH	F: TGCTGCTGGGCTGGTCGATG	[43]
	R: GGGAGGGTGACGGTGACATC
mrkD	F: AAGCTATCGCTGTACTTCCGGCA	[43]
	R: GGCGTTGGCGCTCAGATAGG
entB	F: GTCAACTGGGCCTTTGAGCCGTC	[43]
	R: TATGGGCGTAAACGCCGGTGAT
ybtS	F: GACGGAAACAGCACGGTAAA	[43]
	R: GAGCATAATAAGGCGAAAGA
allS	F: CCGAAACATTACGCACCTTT	[43]
	R: ATCACGAAGAGCCAGGTCAC
kfu	F: GGCCTTTGTCCAGAGCTACG	[43]
	R: GGGTCTGGCGCAGAGTATGC
blaTEM	F: TCGGGGAAATGTGCGCG	[44]
	R: TGCTTAATCAGTGAGGCACC
blaSHV	F: TTAGCGTTGCCAGTGCTC	[45]
	R: GGTTATGCGTTATATTCGCC
blaCTX-M	F: CGCTTTGCGATGTGCAG	[24]
	R: ACCGCGATATCGTTGGT
blaPER	F: CCTGACGATCTGGAACCTTT	[46]
	R: GCAACCTGCGCAATGATAGC
blaVEB	F: CGACTTCCATTTCCCGATGC	[47]
	R: GGACTCTGCAACAAATACGC
blaGES	F: TTGCAATGTGCTCAACGTTC	[48]
	R: TAGTTGTATCTCTGAGGTCG
blaKPC	F: CGTCTAGTTCTGTCTTG	[49]
	R: CTTGTCATCCTTGTTAGGCG
blaVIM	F: GATGGTGTTTGGTCGCATA	[12]
	R: CGAAATGCGCAGCACCAG
blaIMP	F: GGAATAGAGTGGCTTAATTCTC	[48]
	R: CCAAACYACTAAGTTATCT
blaSPM	F: GCCGTTTGAAAATCTGGGTAC	[50]
	R: CCTTCGCTTCAGATCCTCG
blaNDM	F: GGTTTGGCGATCTGGTTTTC	[52]
	R: CGGAATGGCTCATCACGATC
blaOXA-48	F: AAGGAATGGCAAGAAAACAAAA	[51]
	R: CCATAATCGAAAGCATGTAGCA