Research Article

Evaluation of Biodegradable Alloy Fe30Mn0.6N in Rabbit Femur and Cartilage through Detecting Osteogenesis and Autophagy

Figure 9

The results of the fluorescence microscopy analysis: (a) representative immunofluorescence images depicting LC3-II in chondrocytes treated with Fe30Mn0.6N alloy extracts of 0%, 15%, 30%, and 45% for 24 h; the white line represented 20 μm in images; white μm; (b) the graphs represented the quantification of LC3 puncta average number in chondrocyte using ImageJ quantification tool (). Quantification was performed from 3 experiments with >25 cells counted for each condition.
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