Role of Kir4.1 Channels in Aminoglycoside-Induced Ototoxicity of Hair Cells

Figure 3

Concentration conditions of ototoxic drug treatment in HEI-OC1 cells and in the CCK viability assay. Drug concentrations that lead to cell death and cell damage were observed up to day 7 to establish the ototoxic drug dose. (a) Measurement of concentrations of kanamycin and furosemide to determine safe treatment dose. (b) CCK assays performed after 7 days with kanamycin 100 μM and furosemide 10 μM at 39°C and 5% CO₂.

(a)

(b)