Role of Kir4.1 Channels in Aminoglycoside-Induced Ototoxicity of Hair Cells

Figure 4

Association of potassium channels Kir4.1, NKCC1, and KCNQ4 in HEI-OC1 cells. Western blot and qRT-PCR results to determine the association of Kir4.1, NKCC1, and KCNQ4 in HEI-OC1 cells. (a) Treatment of HEI-OC1 cells with either kanamycin or furosemide increased the expression of Kir4.1. (b) When furosemide was treated in HEI-OC1 cells, NKCC1 was blocked, and kanamycin was not affected. (c) When kanamycin was treated in HEI-OC1 cells, KCNQ4 was blocked, and there was no specific effect upon furosemide treatment. (d) Changes in expression levels of potassium channels in ototoxic drug-treated HEI-OC1 cells confirmed by western blotting.

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