Ameliorative Effects of Vitamins A, C, and E on Sperm Parameters, Testis Histopathology, and Oxidative Stress Status in Zinc Oxide Nanoparticle-Treated Rats

Figure 1

Micrographs of testicular tissue from control and treated rats. (a) Control 1 (Bi-distilled water), (b) control 2 (olive oil), (c) Vit A, (d) Vit C, and (e) Vit E show normal histology of the organ showing regular interstitial connective tissue (large arrow) and seminiferous tubules (asterisk) with typical arrangement of spermatogenic cells in various stages of maturation (ellipse). (f) ZnO NP-treated rats (arrowheads, large arrow and small arrow show atrophy of tubular epithelium, sloughing of Sertoli cells, and intratubular empty spaces, respectively.) (g), (h) and (i), respectively, indicate Vit A+ZnO, Vit C+ZnO, and Vit E+ZnO-treated rats so that the disorganized germinal epithelium of seminiferous tubules (arrow) has partly been improved (×100 magnification (inset ×1000), hematoxylin and eosin).

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