Research Article

Inflammatory and Oxidative Stress Biomarkers in the Elderly, the Birjand Longitudinal Aging Study

Table 1

Oxidant stress measurement.

TestMethodType of kiteCompanyApparatus

PPPHRadical scavenging activity (RSA) based on DPPH assay)Microplate colorimetry (Zantox)Kavosh Arian Azma Co.Microplate reader, Biotek instruments (Epock, USA)
ThiolTotal thiol groups based on Ellman assayMicroplate colorimetry (Zantox)Kavosh Arian Azma Co.Microplate reader, Biotek instruments (Epock, USA)
TBARsLipid peroxidation based on Thiobarbituric acid reactive substancesFlourometry (Zantox)Kavosh Arian Azma Co.Microplate reader, Bioteck Life Science instrumentations, Citation3 imaging reader (Vermont, USA)
FRAPTotal antioxidant capacity (TAC) based on FRAP assayMicroplate colorimetry (Zantox)Kavosh Arian Azma Co.Microplate reader, Biotek instruments (Epock, USA)