Circular RNA circ_0026218 Suppressed Atherosclerosis Progression via miR-338-3p/SIRT6 Axis

Figure 1

Downregulation of circ_0026218 expression in HUVECs with ox-LDL. (a) As the cutoff criteria ( >1 and ), the volcano plot was used to analyze and screen out the circRNAs that were significantly up- and downregulated in the AS cell model induced by ox-LDL. Blue represents downregulated circRNAs, and black represents circRNAs that were not significantly differentially expressed. (b, c) circ_0026218 expression in HUVECs, stimulated with ox-LDL at discrepant concentrations (0 μg/mL, 50 μg/mlL, 75 μg/mL, or 100 μg/mL) and periods (0 h, 24 h, 48 h, and 72 h), were, respectively, probed by qRT-PCR. (d) circ_0026218 and GAPDH expressions, after the total RNA from HUVECs were incubated with RNase R, were exposed by qRT-PCR. (e) circ_0026218, U6, and GAPDH expressions in the nucleus and cytoplasm of HUVECs were subjected to qRT-PCR. , , .

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