Evaluation of Systemic Genotoxic/Oxidative and Proinflammatory Effects in Workers of a Titanium Dioxide Production Plant

Table 1

Analysis of human polymorphic genes in the Caucasian population.

Gene polymorphism	rs number	Primer sequence	Annealing temperature	Restriction enzyme pattern	Results	Reference
GST-T1	rs17856199	F: 5-TTC CTT ACT GGT CCT CAC ATC TC-3	62°C	Absence of restriction pattern	Positive/null	Teixeira, J.P., et al. Toxicology. 2004; 195 (2-3), 231-242
		R: 5-TCA CCG GAT CAT GGC CAG CA-3
GST-M1	rs366631	F: 5-GAA CTC CCT GAA AAG CTA AAG C-3	62°C	Absence of restriction pattern	Positive/null	Sørensen M. et al. Int J Cancer. 2004; 110(2):219-224
		R: 5-GTT GGG CTC AAA TAT ACG GTG G-3
hOGG1 326	rs1052133	F: 5-GGA AGG TGC TTG GGG AAT-3	58°C	Fnu4HI	Wild type: 200 bp; heterozygous: 200 bp, 100 bp; mutant: 100 bp	Le Marchand et al. Cancer Epidemiology, Biomarkers and Prevention. 2002; 11 (4):409-412
		R: 5-ACT GTC ACT AGT CTC ACC AG-3
XRCC1 399 G/A	rs 25487	F: 5-TTG TGC TTT CTC TGT GTC CA-3	62°C	MSPI	Wild type: 374 and 221 bp; heterozygous: 615 bp, 374 and 221 bp; mutant: 615 bp	Kowalski et al. Journal of Experimental and Clinical Cancer Research. 2009; 28 : 37
		R: 5-TCC TCC AGC CTT TTC TGA TA-3

XRCC1: X-ray repair cross-complementing group 1; hOGG1: 8-oxoguanine DNA glycosylase-1; GST: glutathione S-transferase.