Blockade of EP4 by ASP7657 Modulates Myeloid Cell Differentiation In Vivo and Enhances the Antitumor Effect of Radiotherapy

Figure 1

Effect of ASP7657 on PGE₂-mediated suppression of the differentiation of mouse BM cells and hCD14⁺ monocytes into DCs in vitro: (a, c) representative flow cytometry dot plots; (b, d) effect of ASP7657 on the DC population; (a, b) mouse BM cells were cultured with 10 ng/mL mGM-CSF in the presence or absence of 10 nM PGE₂ for 7 days; (c, d) human CD14⁺ monocytes were cultured with 50 ng/mL hGM-CSF and 50 ng/mL hIL-4 in the presence or absence of 10 nM PGE₂ for 7 days; (a, c) PGE₂ suppressed differentiation of mouse BM cells and human CD14⁺ monocytes into DCs in vitro; (b, d) addition of ASP7657 throughout the culture period reversed PGE₂-mediated suppression of DC differentiation at the indicated doses. Shown are representative results of 2 independent assays.

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