Phytochemical Study and In Vitro Biological Activities of Hibiscus panduriformis Burm. f. (Malvaceae), Alternanthera pungens Kunth (Amaranthaceae), and Wissadula rostrata (Schumach.) Hook. f. (Malvaceae)

Table 3

DPPH and ABTS radical scavenging effect of different organic extracts of H. panduriformis, A. pungens, and W. rostrata.

Sample	DPPH IC50 (mg/mL)	ABTS IC50 (mg/mL)
H. pan-Hex	> 1^a	> 4^a
H. pan-DCM	> 1^a	> 4^a
H. pan-EtOAc	> 1^a	> 4^a
H. pan-MeOH	^b	^b
A. pun-Hex	> 1^a	> 4^a
A. pun-DCM	> 1^a	> 4^a
A. pun-EtOAc	^c	> 4^a
A. pun-MeOH	^d	^c
W. ros-Hex	> 1^a	> 4^a
W. ros-DCM	> 1^a	> 4^a
W. ros-EtOAc	^e	> 4^a
W. ros-MeOH	^f	^d
Trolox	^g	^e

Values are presented as , and each extract was tested in triplicate. For each test (DPPH and ABTS) and each plant, extracts were compared regarding the organic solvent (two-way ANOVA followed by Tukey’s test) and Trolox (two-way ANOVA followed by Dunnett’s test). For the DPPH test, values bearing different letters are considered highly statistically significant at ^{(a vs. b, a vs. c, a vs. d, a vs. e, a vs. f, a vs. g, b vs. c, b vs. d, b vs. e, b vs. f, b vs. g, c vs. d, c vs. e, c vs. f, c vs. g, d vs. e, d vs. f, d vs. g, e vs. f, e vs. g, and f vs. g)}. For the ABTS test, values bearing different letters are considered highly statistically significant at ^{(a vs. b, a vs. c, a vs. d, a vs. e, b vs. e, c vs. d, c vs. e, and d vs. e)}, at ^{(b vs. c)}, and at ^{(b vs. d)}. H. pan: Hibiscus panduriformis; A. pun: Alternanthera pungens; W. ros: Wissadula rostrata; Hex: hexane extract; DCM: dichloromethane extract; EtOAc: ethyl acetate extract; MeOH: methanol extract.