Research Article
Characterization and Analysis of HEK293/Adenovirus Type 5 Cell Cultures under Simulated Microgravity Using Differential Neural Network Modeling
Figure 1
Determination of HEK293 cell gravity percentage under SMGC. (a) Trajectory plot of HEK293 cells taken from digital processing of photograms at an agitation speed of 25 rpm. (b) Representation of velocity vector of the HEK293 cell spheroid. The calculated average value for velocity vector magnitude was m/s S.D. 0.0026 m/s. (c) Representation of acceleration vector of the HEK293 cell spheroid. The calculated average value for the acceleration vector magnitude was m/s2 S.D. 0.0204 m/s2. Considering acceleration on -axis, minimum and maximum values were m/s2 and m/s2 (minus sign indicates downward direction); the percentage of gravity of this minimum and maximum vertical acceleration perceived by the HEK293 cells in the HARV culture system were (downward direction) and (where minus sign implies against the gravity direction); this is the fraction of gravity considering m/s2. The effect of gravity is minimum in the downward direction, compensated for the rotational system.
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