Characterization and Analysis of HEK293/Adenovirus Type 5 Cell Cultures under Simulated Microgravity Using Differential Neural Network Modeling

Figure 2

(a) Growth of HEK293 cell culture under SMGC. (A) Growth kinetics of HEK293 cell cultures in the serum-free medium under SMGC and EGC. The doubling time (dt) for μg cultures was 31 h compared to 28 h for EGC in the shake flask. (B) Estimation of HEK293 cell culture growth using a DNN from experimental data. (C) Estimation of cell growth productivities for SMGC and EGC calculated from the DNN approach. Maximum cell growth productivities are observed between 48 and 72 h, after which a decrease in growth rate is observed for both conditions. (b) Morphology of HEK293 cell cultures at 48 h, 96 h, and 144 h 20x. (A) Static HEK293 cell culture in serum-free medium under EGC 20x. Scale bar corresponds to 200 microns (red). (B) HEK293 cell culture in the shake flask in the serum-free medium under EGC. Scale bar corresponds to 100 microns (red). (C) HEK293 cell culture in serum-free medium in HARV bioreactor under SMGC. The scale bar corresponds to 200 microns (red). An evident morphological change is observed. SMGC in the serum HARV bioreactor allows the development of suspension cultures where the cells are spherical without aggregate formation. The EGC in static cultures promotes cell adhesion while in shaken flask cultures the formation of cell aggregates is observed.

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