Characterization and Analysis of HEK293/Adenovirus Type 5 Cell Cultures under Simulated Microgravity Using Differential Neural Network Modeling

Figure 3

Production of Ad5/g-inf in 293HEK cells under SMGC. (a) Titer of infectious viral particles determined by end-point dilution at 72 h postinfection and % of viability. (b) Estimation of viral titer by a differential neural network (DNN) in HEK293 cell cultures from experimental data. (c). Volumetric production rate of adenoviral particles. As previously reported, the production of adenoviral particles starts after 24 h postinfection in both conditions. The SMGC favored the viral vector production by two exponential values reflecting the value in the volumetric production rate which maximum value was reached at 81.1 h in comparison to the EGC value of 96 h postinfection.

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