Characterization and Analysis of HEK293/Adenovirus Type 5 Cell Cultures under Simulated Microgravity Using Differential Neural Network Modeling

Figure 4

Production of recombinant gamma interferon in 293HEK cells under SMGC. (a) Recombinant gamma interferon concentration determined from supernatants at 72, 96, and 120 h. (b) DNN approach of recombinant gamma interferon concentration with corresponds to the observed experimental data. (c) Gamma interferon volumetric production rates. It is observed that the SMGC predicts a higher volumetric production rate at SMGC (up to 0.11 pg/mLh for the HARV culture at 120 h postinfection) compared to the EGC values (up to 0.01 pg/mLh for the shake flask). As observed, no significant difference in concentration was obtained between both conditions, but there is a trend to increase the recombinant protein production in the case SMGC.

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