Cystic Fibrosis Mice Are Highly Susceptible to Repeated Acute Pseudomonas aeruginosa Pneumonia after Intranasal Inoculation

Figure 2

Lung leukocyte counts in CF and non-CF mice. Lung leukocyte counts were examined at baseline, at 4 hours after the one-time instillation, at 6 days after the instillation, and at 4 hours after the second instillation of Pseudomonas aeruginosa (PA) in CF and non-CF mice using flow cytometry analysis. (a) Neutrophils, (b) macrophages, (c) NK cells, (d) B cells, (e) CD4 T cells, and (f) CD8 T cells were probed using Ly6G, F4/80, NK1.1, B220, CD4, and CD8 antibodies, respectively. Data are shown as ( per arm). One-way ANOVA with the Bonferroni post hoc analysis was performed. .

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