Cystic Fibrosis Mice Are Highly Susceptible to Repeated Acute Pseudomonas aeruginosa Pneumonia after Intranasal Inoculation

Figure 6

Single-cell RNA sequencing analysis of lung cells and neutrophil DEG/ontology. (a) Naïve CF and non-CF lung cells and CF and non-CF lung cells post two-time instillation were subjected to single-cell RNA sequencing analysis. Annotation results are shown. (b) Heatmap of lung neutrophil differentially expressed genes (DEGs). (c) The number of up- and downregulated DEGs. (d) Upregulated DEGs in CF and non-CF lung neutrophils were subjected to KEGG pathway analysis and presented as bubble enrichment maps. (e) Downregulated DEGs in CF and non-CF lung neutrophils were subjected to KEGG pathway analysis and presented as bubble enrichment maps. (f) Adhesion molecule expression pattern was shown on the heatmap. (g) tlr4 expression level was shown in violin plots.

(a)

(b)

(c)

(d)

(e)

(f)

(g)