In Silico Identification and Characterization of Drug Targets in Streptococcus pneumoniae ATCC 700669 (Serotype 23F) by Subtractive Genomics

Table 2

Essential, nonhomologous, and nonparalogous proteins in unique pathways of S. pneumoniae 23F (https://www.uniprot.org).

S/N	Gene name	Protein name	UniProt KB ID	Subcellular location	Functions of the proteins
1	asd	L-aspartate-semialdehyde dehydrogenase	B8ZPG6	Cytoplasmic	Isoleucine, lysine, and threonine biosynthetic process, “de novo” L-methionine biosynthetic process
2	pyrH	Aspartate kinase	B8ZLI0	Cytoplasmic	Phosphoribosylaminoimidazolecarboxamide formyltransferase activity, de novo inosine monophosphate biosynthetic process
3	dapB	4-hydroxy-tetra hydrodipicolinate reductase	B8ZLL9	Cytoplasmic	Catalyzes the conversion of 4-hydroxy-tetra hydrodipicolinate to tetra hydrodipicolinate; Lysine biosynthesis process
4	MurF	UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase	B8ZM65	Cytoplasmic	ATP binding, cell cycle, peptide glycan biosynthetic process
5	murZ	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	B8ZPT0	Cytoplasmic	Catalysis of the reaction: phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxy vinyl)-D-glucosamine
6	murB	UDP-N-acetylmuramate dehydrogenase	B8ZKN7	Cytoplasmic	Peptidoglycan biosynthetic process (uridine diphospho-N-acetyl glucose amine enolpyruvate reductase activity)
7	MurC	UDP-N-acetylmuramate--alanine ligase	B8ZLC2	Cytoplasmic	Cell wall formation; UDP-N-acetylmuramate-L-alanine ligase activity, peptidoglycan biosynthesis
8	MurD	UDP-N-acetylmuramoyl alanine-D-glutamate ligase	B8ZMZ6	Cytoplasmic	Cell wall formation, catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetyl muramoyl-L-alanine, peptidoglycan biosynthesis
9	ddl	D-alanine-D-alanine ligase	B8ZM66	Cytoplasmic	Cell wall formation, D-alanine-D-alanine ligase activity, ATP binding, cell wall organization, peptidoglycan biosynthesis, regulation of cell shape
10	uppP	Undecaprenyl-diphosphatase	B8ZLV4	Cytoplasmic	Confers resistance to bacitracin, response to antibiotic, and involved in peptidoglycan biosynthetic process
11	pbpX	Penicillin-binding protein 2X	Q4TUL5	Cytoplasmic membrane	Binding to penicillin, an antibiotic that contains the condensed beta-lactam thiazolidine ring system.
12	murE	UDP-N-acetyl muramoyl-L-alanyl-D glutamate-L-lysine ligase	B8ZLD0	Cytoplasmic	Cell division, cell cycle, ATP binding activity, cell wall organization
13	SP_1589	Lipid II isoglutaminyl synthase (glutamine-hydrolysing)	B8ZM05	Cytoplasmic	Carbon-nitrogen ligase activity on lipid II, peptidoglycan biosynthetic process, cell wall organization
14	MurM	Serine/alanine adding enzyme	B8ZMK2	Cytoplasmic	Aminoacyltransferase activity, cell wall macromolecule biosynthetic process
15	Fba	Fructose-bisphosphate aldolase, class II	B8ZMD4	Cytoplasmic	Zinc ion binding, fructose 1,6-bisphosphate metabolic process, glycolytic process
16	rpoD	RNA polymerase primary sigma factor	B8ZPS2	Cytoplasmic	DNA binding, sigma factor activity, DNA-templated transcription initiation
17	secG	Preprotein translocase subunit SecG	B8ZP70	Cytoplasmic membrane	Involved in protein export. Participates in an early event of protein translocation, protein secretion, protein transporting ATPase activity
18	YesM	Two-component system, sensor histidine kinase YesM	B8ZKC2	Cytoplasmic membrane	Protein-transporting ATPase activity, protein transport by the Sec complex
19	PhoP	Two-component system, OmpR family, alkaline phosphatase synthesis response regulator	B8ZPK5	Cytoplasmic	Regulation of DNA-templated transcription, transcription cis-regulatory region binding, phosphorelay response regulator activity
20	alr	Alanine racemase	B8ZMG1	Cytoplasmic	Catalyzes the interconversion of L-alanine and D-alanine. Pyridoxal phosphate binding, D-alanine biosynthetic process
21	murG	UDP-N-acetylglucosamine-N-acetyl muramyl pyro phosphoryl-undecaprenol N-acetylglucosamine transferase	B8ZMZ7	Cytoplasmic membrane	Cell wall formation, carbohydrate metabolic process