Citrus limon (L.) Osbeck Fruit Peel Extract Attenuates Carbon Tetrachloride-Induced Hepatocarcinogenesis in Sprague-Dawley Rats

Figure 3

Effect of LPE treatment on periodic acid Schiff- (PAS-) stained liver parenchyma. (a) Control group, (b) model group, (c) capecitabine group, (d) LPE (50 mg/kg po), (e) LPE (100 mg/kg po), and (f) LPE (200 mg/kg po). (I) Liver parenchyma. (II) Centrilobular hepatocytes. (III) Periportal hepatocytes. The control group received olive oil (5 mL/kg ip) in the morning twice a week for 16 weeks. The model group received CCl₄/olive oil (2 mL/kg ip) in the morning twice a week for 16 weeks. LPE and capecitabine groups received CCl₄/olive oil (2 mL/kg ip) in the morning and, respectively, LPE (50, 100, and 200 mg/kg po) and capecitabine (100 mg/kg po) in the afternoon twice a week for 16 weeks. Black arrows represent stromal invasion; red arrows represent hepatocytes exhibiting lipid accumulation; yellow arrows represent liver parenchyma where hepatocytes do not exhibit lipid accumulation. LPE: Citrus limon fruit peel extract.