Citrus limon (L.) Osbeck Fruit Peel Extract Attenuates Carbon Tetrachloride-Induced Hepatocarcinogenesis in Sprague-Dawley Rats

Figure 4

Semiquantitative analysis of glycogen content of hepatocytes. (a) Image processing workflow of micrographs of periodic acid Schiff- (PAS-) stained liver photomicrographs for glycogen quantification. (A) control group, (B) model group, (C) capecitabine group, (D) LPE (50 mg/kg po), (E) LPE (100 mg/kg po), and (F) LPE (200 mg/kg po). (b) Bar graph showing percent threshold for PAS-stained glycogen content in each group. Original micrograph (I). (II, III) Processed images (PAS and hematoxylin, respectively) after color deconvolution using user-defined vectors (VI). (IV) The inverted forms of (II). The control group received olive oil (5 mL/kg ip) in the morning twice a week for 16 weeks. The model group received CCl₄/olive oil (2 mL/kg ip) in the morning twice a week for 16 weeks. LPE and capecitabine groups received CCl₄/olive oil (2 mL/kg ip) in the morning and, respectively, LPE (50, 100, and 200 mg/kg po) and capecitabine (100 mg/kg po) in the afternoon twice a week for 16 weeks. ×400. Each value is the , , , control vs. model, ; ^#, model vs. LPE, . LPE: Citrus limon fruit peel extract.

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