Two Main Cancer Biomarkers as Molecular Targets of Binase Antitumor Activity

Figure 6

The effect of binase on EGF-stimulated MAPK phosphorylation in BT-20 cells. (a) Cells were stimulated with EGF alone or in combination with binase for 30 min on a serum-starved medium. (b) Cells were stimulated with EGF for 30 min on a serum-starved medium with and without their pretreatment with binase for 30 min. (c) Cells were stimulated with EGF for 30 min on serum-starved medium followed by treatment with binase for 30 and 90 min. Data represent the fold change of fluorescence intensity of binase-treated cells as compared to the binase-untreated cells. Significant differences are indicated as , , and .

(a)

(b)

(c)