Effect of DNMT3A R882H Hot Spot Mutations on DDX43 Promoter Methylation in Acute Myeloid Leukemia

Figure 1

Tetra ARMS-PCR. Patients 23 and 29 are heterozygous in R882H of DNMT3A gene (2 out of 5 patients have been shown in the picture, P23 and P29). They showed 3 bounds in 160, 214, and 317 bp. Patients 33 and 31 are wild-type homozygous without mutation in R882H. They have only 2 bounds in 160 and 317 bp (2 out of 42 have been shown here, P31 and P33). 100 bp ladders were used in 1.5% agarose gel. P: patient; Neg: negative.