Effect of DNMT3A R882H Hot Spot Mutations on DDX43 Promoter Methylation in Acute Myeloid Leukemia

Figure 3

(a) Amplification curves of patients’ samples. (b) Normalized melting curve of DDX43 serial standard dilution including 0, 25, 50, 75, and 100. Sample with R882H mutation (R882H+) showed methylation between 25 and 50% methylation. Sample without R882H mutation (R882H-) showed methylation between 75 and 100% methylation. (c) Differential graph of each standard sample against the unmethylated sample. Methylation range between R882H+ sample and R882H- sample was compared to the other standard sample.

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