The Improvement of Sepsis-Associated Encephalopathy by P2X7R Inhibitor through Inhibiting the Omi/HtrA2 Apoptotic Signaling Pathway

Figure 3

Effects of LPS or A-438079 on ROS production and mitochondrial membrane potential in HBMECs in vitro. (a) The adherent HBMECs in the group were treated with A-438079 for an hour and then treated with LPS for 12 hours. The same cells in the group were treated with DMSO for an hour and then treated with LPS for 12 hours. The LPS group was treated with LPS one hour after the addition of the same amount of normal saline for 12 hours. Mito-tracker Red CMXROS was used to evaluate the mitochondrial membrane potential in each group, and the intensity of red fluorescence signal in each group indicated the change of mitochondrial membrane potential. . (b) A fluorescence microscope was used to observe the production of ROS in each group. . (c) The fluorescence signals of different groups were analyzed using ImageJ. All data are expressed as . vs. control group, ^# vs. LPS group, and ^@ vs. group.

(a)

(b)

(c)