Vitamin B6 Deficiency Induces Autism-Like Behaviors in Rats by Regulating mTOR-Mediated Autophagy in the Hippocampus

Figure 2

VB₆ deficiency reduced GABAergic inhibition in hippocampal CA3 area of offspring rats. The offspring rats obtained from their pregnant mothers with VB₆ normality, VB₆ deficiency, or VB₆ supplement were modeled to evaluate autism-like behaviors. Hippocampal tissues were collected after euthanasia of offspring rats. The expressions of VIAAT and GAD67 in the hippocampal CA3 area of rat were determined using immunofluorescence staining (×600 magnification, scale bar: 40 μm). Three rats/group. One-way analysis of variance with Tukey’s post-hoc test. VB₆: vitamin B6; VIAAT: vesicular inhibitory amino acid transporter; GAD67: glutamate decarboxylase 67.