Vitamin B6 Deficiency Induces Autism-Like Behaviors in Rats by Regulating mTOR-Mediated Autophagy in the Hippocampus

Figure 3

VB₆ deficiency diminished GAD67⁺ inhibitory synapses in hippocampal CA3 area of offspring rats. The offspring rats obtained from pregnant mothers with VB₆ normality, VB₆ deficiency, or VB₆ supplement were modeled. Hippocampal tissues were collected after euthanasia. Immunofluorescence analysis was used to detect synaptic inhibition of neurons in the hippocampus by staining GAD67⁺ and vGAT (×400 magnification, scale bar: 50 μm). Three rats/group. One-way analysis of variance with Tukey’s post-hoc test. VB₆: vitamin B6; GAD67⁺: glutamate decarboxylase 67-positive; vGAT: vesicular gamma-aminobutyric acid transporter.