Research Article

AP-1 as a Regulator of MMP-13 in the Stromal Cell of Giant Cell Tumor of Bone

Figure 5

The effect of siRNA knockdown on the level of MMP-13 activity in IL-1β-stimulated GCT cells. Filtered and concentrated conditioned media from siRNA silenced GCT stromal cells were analyzed using the MMP-13 activity kit as per optimization in our lab. Results of the ELISA activity assay are shown in triplicate with error bars. The MMP-13 activity levels were normalized to the amount of total protein for each condition.
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