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Biochemistry Research International
/
2011
/
Article
/
Tab 1
/
Review Article
Bioseparation of Four Proteins from
Euphorbia characias
Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase
Table 1
Purification of
Euphorbia
latex peroxidase.
Step
Total protein (mg)
Total activity (nkat)
Specific activity (nkat/mg)
Yield (%)
Purification (fold)
Acetone powder crude extract
3500
71545
20.4
100
1
Ammonium sulfate fractionation
534
40910
76.6
57
3.7
DEAE-cellulose chromatography
20.3
33414
1646
46.7
80.7
Hydroxylapatite column chromatography
5.2
28455
5500
39.8
270