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Biochemistry Research International
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2011
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Article
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Tab 2
/
Review Article
Bioseparation of Four Proteins from
Euphorbia characias
Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase
Table 2
Purification of
Euphorbia
latex nucleotide pyrophosphatase/phosphodiesterase.
Step
Total protein (mg)
Total activity (nkat)
Specific activity (nkat/mg)
Yield (%)
Purification (fold)
Acetone powder crude extract
3500
9500
2.7
100
1
Controlled heating (60°C)
360
6665
18.5
70
6.8
Ammonium sulfate fractionation
210
5615
26.7
59
10
DEAE-cellulose chromatography
53
3780
71.3
40
26