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Biochemistry Research International
/
2011
/
Article
/
Tab 4
/
Review Article
Bioseparation of Four Proteins from
Euphorbia characias
Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase
Table 4
Purification of
Euphorbia
latex copper amine oxidase.
Step
Total protein (mg)
Total activity (nkat)
Specific activity (nkat/mg)
Yield (%)
Purification (fold)
Acetone powder crude extract
3500
37917
10.8
100
1
Ammonium sulfate fractionation
534
33817
63
89.2
5.8
DEAE-cellulose chromatography
200.3
30000
150
79
13.8
Hydroxylapatite column chromatography
80.2
28067
350
74
32
-Aminohexy-Sepharose 4B column chromatography
41
23233
567
61
52.5