Bioseparation of Four Proteins from <i>Euphorbia characias</i> Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase

<table class="table-group" id="tab5"><tr><td><table class="table"><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr class="thead"><td align="left">Step</td><td align="center">Total protein  (mg)</td><td align="center">Total activity (nkat)</td><td align="center">Specific activity (nkat/mg)</td><td align="center">Yield (%)</td><td align="center">Purification (fold)</td></tr><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr><td align="left">Acetone powder crude extract</td><td align="center">3500</td><td align="center">71545</td><td align="center">20.4</td><td align="center">100</td><td align="center">1</td></tr><tr><td align="left">Ammonium sulfate fractionation</td><td align="center">534</td><td align="center">40910</td><td align="center">76.6</td><td align="center">57</td><td align="center">3.7</td></tr><tr><td align="left">CaM-sepharose column chromatography</td><td align="center">5.9</td><td align="center">33536</td><td align="center">5700</td><td align="center">46.8</td><td align="center">279</td></tr><tr class="table-tr"><td colspan="6"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Purification of <i>Euphorbia</i> latex peroxidase by affinity chromatography.

Biochemistry Research International

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Table 5

Table 5: Bioseparation of Four Proteins from <i>Euphorbia characias</i> Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase 

Table 5 | Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase 