miR-142-5p as a CXCR4-Targeted MicroRNA Attenuates SDF-1-Induced Chondrocyte Apoptosis and Cartilage Degradation via Inactivating MAPK Signaling Pathway

Figure 1

Screening and identification of candidate miRNAs involved in the SDF-1/CXCR4 axis in OA pathogenesis through miRNA microarray and bioinformatics analyses (a, b). The aberrantly expressed miRNAs were screened out by miRNA microarray analyses of cartilage tissue samples from OA patients and healthy cartilage tissue samples. Top downregulated miRNAs (a) and upregulated miRNAs (b) in OA cartilage tissues are shown. (c) CXCR4 expression in SDF-1-induced human chondrocytes by immunohistochemistry staining. Scale bar, 50 μm. (d) Quantification of relative CXCR4 expression in chondrocytes shown in (c). (e) The levels of four most downregulated and putative CXCR4-targeted miRNAs in control and SDF-1-stimulated OA human chondrocytes (100 ng/ml, for 24 h) were determined by qRT-PCR. n = 8 for each group. Significant differences are indicated as and . miRNAs, microRNAs; SDF-1, stromal cell-derived factor 1.

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