The Stability Improvement of Aspergillus fumigatus α-Amylase by Immobilization onto Chitin-Bentonite Hybrid

Table 1

K_M and values for free and immobilized enzymes.

Sample	(μmole mL^−1 min^−1)	KM (mg mL^−1 substrate)
Free enzyme	10.90 ± 1.8940	3.04 ± 1.0430
Immobilized enzyme	3.37 ± 0.1159	11.57 ± 0.7569

The kinetic parameters were determined at each optimum temperature and the starch concentrations from 2.0 to 10.0 mg/mL. The values were shown as mean ± SD, n = 2.