Diets Rich in Olive Oil, Palm Oil, or Lard Alter Mitochondrial Biogenesis and Mitochondrial Membrane Composition in Rat Liver
Table 5
Variation in mitochondrial enzyme activity.
Control
Crude PO
Refined PO
OO
Lard
Complex I (mU/mg protein)
1.56 ± 0.25
1.31 ± 0.16
1.40 ± 0.14
1.41 ± 0.14
1.42 ± 0.11
NS
Complex II (mU/mg protein)
1.85 ± 0.05a
1.74 ± 0.04ab
1.80 ± 0.08a
1.57 ± 0.07b
1.70 ± 0.07ab
0.028
Complex II + III (mU/mg protein)
0.078 ± 0.006
0.077 ± 0.005
0.094 ± 0.010
0.065 ± 0.007
0.069 ± 0.007
NS
Complex IV (mU/mg protein)
0.796 ± 0.086
0.767 ± 0.090
0.846 ± 0.129
0.819 ± 0.097
0.902 ± 0.058
NS
Citrate synthase (mU/mg protein)
96.9 ± 6.12
103 ± 7.12
101 ± 6.68
106 ± 5.12
104 ± 6.72
NS
CLS (WB)
1.00 ± 0.22a
0.64 ± 0.84a
0.63 ± 0.90a
0.52 ± 0.12b
0.38 ± 0.84b
0.023
Values are expressed as mean ± SD (n = 7-8). On the same line, values with different letters are significantly different. CLS, cardiolipin synthase; WB, western blot.