miR-21 Regulates Immune Balance Mediated by Th17/Treg in Peripheral Blood of Septic Rats during the Early Phase through Apoptosis Pathway

Figure 5

The ratios of Th17 and Treg cells in peripheral blood were changed after transfection of miR-21 activator or inhibitor into lymphocytes of septic rats. ((a) and (c)) The ratios of Th17 cells in peripheral blood in eight-paired NC rat group, SP rat group, SP rat + miR21 mimic group, and SP rat + miR21 inhibitor group were determined by flow cytometry [t value (NC versus SP) = 18.445,  < 0.01; t value (miR-21 mimic versus SP) = 6.362,  < 0.01; t value (miR-21 inhibitor versus SP) = 2.477,  < 0.05]. ((b) and (d)) The ratios of Treg cells in peripheral blood in eight-paired NC rat group, SP rat group, SP rat + miR21 mimic group, and SP rat + miR21 inhibitor group were determined by flow cytometry [t value (NC versus SP) = 9.836,  < 0.01; t value (miR-21 mimic versus SP) = 5.991,  < 0.01; t value (miR-21 inhibitor versus SP) = 5.180,  < 0.01]. NC group: normal control group; SP group: sepsis group; SP rat + miR21 mimic group: sepsis group with miR-21 activator transfected into lymphocytes; SP rat + miR21 inhibitor group: sepsis group with miR-21 inhibitor transfected into lymphocytes.  < 0.01.

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