Mass Spectrometry of Putrescine, Spermidine, and Spermine Covalently Attached to Francisella tularensis Universal Stress Protein and Bovine Albumin

Table 1

Neutral masses of polyamine adducts on glutamate, glutamine, and aspartate.

Polyamine	Amino acid residue	Catalyst	Dehydro residue	Polyamine added mass	Adduct neutral mass
Putrescine	Glutamate	EDC	129.04	70.06	199.10
Putrescine	Glutamine	Transglutaminase	128.05	71.07	199.12
Spermidine	Glutamate	EDC	129.04	127.12	256.16
Spermidine	Glutamine	Transglutaminase	128.05	128.13	256.18
Spermine	Glutamate	EDC	129.04	184.18	313.22
Spermine	Glutamine	Transglutaminase	128.05	185.19	313.24
Putrescine	Aspartate	EDC	115.02	70.06	185.08
Spermidine	Aspartate	EDC	115.02	127.12	242.14
Spermine	Aspartate	EDC	115.02	184.18	299.20

There are no entries in Table 1 for transglutaminase adducts on glutamate or aspartate because transglutaminase only labels glutamine.