Long Noncoding RNA TUG1 Promotes the Function in ox-LDL-Treated HA-VSMCs via miR-141-3p/ROR2 Axis

Figure 3

TUG1 was a direct target of miR-141-3p. (a) The putative binding sites between miR-141-3p and TUG1 were predicted by starBase. (b) The predicted sites were identified by dual-luciferase reporter assay. The ox-LDL-stimulated HA-VSMCs were transfected with pcDNA-TUG1 or negative control. (c, d) qRT-PCR was used to detect the level of TUG1 (c) or miR-141-3p (d) in each group. (f–j) The ox-LDL-administrated HA-VSMCs were transfected with sh-TUG1+anti-miR-141-3p or sh-TUG1+anti-miR-NC for further experiments. (f) MTT assay was conducted to evaluate the cell viability. (g) The proliferation-associated protein levels of Ki-67 and CyclinD1 were confirmed by western blot. (h, i) The cell migratory and invasive abilities were evaluated by transwell assay. (j) The expression of metastasis-associated proteins, β-catenin, and Vimentin was detected by western blot assay. .

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