Wogonin Inhibits Cardiac Hypertrophy by Activating Nrf-2-Mediated Antioxidant Responses

Figure 2

Wog inhibited oxidative stress in Ang II-induced H9C2 cells. H9C2 cells were pretreated with Wog (20 μM) for 2 hours and then incubated with AngII (1 μM) for the times indicated. (a, b) Representative images of DHE staining from H9C2 cells (magnification: ×200, scale bar: 20 μm); (c) MDA concentration (); (d) SOD activity (); (e–h) mRNA expression of Nrf-2, Keap-1, Ho-1, and Nqo-1 by RT-qPCR (); (i–k) Western blot analysis of Nrf-2 and Keap-1 in H9C2 cells. GAPDH was used as the loading control (). , , and compared to Ctrl; , , and compared to AngII.

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